Fast and deep phosphoproteome analysis with the Orbitrap Astral mass spectrometer
Abstract Owing to its roles in cellular signal transduction, protein phosphorylation plays critical roles in myriad cell processes.That said, detecting and quantifying protein phosphorylation has remained a challenge.We describe the u11-200ps use of a novel mass spectrometer (Orbitrap Astral) coupled with data-independent acquisition (DIA) to achie